Journal: NPJ Vaccines
Article Title: Characterization of dual DNA polymerase knockout strains of Candida albicans with live whole-cell vaccine competence
doi: 10.1038/s41541-025-01291-x
Figure Lengend Snippet: A A liquid growth curve was carried out from the overnight grown cultures of WT (Pink), rad30 ΔΔ (purple), pol32 ΔΔ (green), rad30 ΔΔ pol32 ΔΔ (blue), and pol32 ΔΔ rad30 ΔΔ (orange) in 10 mL YPD broth. Absorbance was measured at 600 nm wavelength for 14 h at an interval of 2 h. The experiments were performed twice with technical triplicates and the result was represented as mean ± SEM. The OD values were plotted using GraphPad Prism 8.0. B Susceptibility of the above-mentioned strains to (i) temperature (16 °C, 30 °C, 37 °C and 42 °C), (ii) HU, (iii) MMS, (iv) TBHP, (v) Cisplatin and (vi) UV by spot dilution assay. The well-grown culture plates were photographed in the ChemiDoc image system.
Article Snippet: YPD media (Cat# 4001032), Maltose (Cat#102241), Hydroxyurea (Cat#102023), Cisplatin (Cat#198872), and MMS (Cat#205518) were procured from MP Biomedicals, USA.
Techniques: Dilution Assay